个人简历

杨丽，女。东北师范大学化学学院，教授，博士生导师。2002年毕业于中国科学技术大学，获博士学位，其后在欧洲和美国研究机构从事博士后研究工作，2008年以优秀学术骨干引进东北师范大学。主要研究方向为发展以毛细管电泳/电色谱为基础的复杂样品和痕量物质的分离分析技术。至今已在Anal. Chem., ACS Sensors，Biosens. Bioelectron., Anal. Chim. Acta,Talanta, J. Chromatgr. A等刊物上发表SCI 收录论文80余篇，主持国家基金委、教育部、吉林省科技厅等多项科研项目。 研究方向： 新型分离分析技术研究与应用 受教育经历： 1998/09 - 2002/06，中国科学技术大学，化学系，博士 1997/09 - 1998/06，中国科学技术大学，化学系，硕士 1993/09 - 1997/06，中国科学技术大学，应用化学系，学士 研究工作经历： 2014/02 - 至今， 东北师范大学，化学学院，教授，博士生导师 2008/02 - 2014/2， 东北师范大学，化学学院，副教授，博士生导师 2006/07 - 2007/12，美国Calibrant Biosystem，Inc. & 美国马里兰大学，博士后 2005/04 - 2006/06，美国路易斯安那州立大学，化学系，博士后 2003/07 - 2004/10，爱尔兰科克大学，化学系，博士后 教学任务： 高等分析化学 分析化学 化合物成分分析实验 Chromatography （留学生研究生课程） 科研项目： 13. 国家自然科学基金面上项目，22074014、“集成多金属氧簇化合物微反应器的新型微通道实时在线分析系统的研究与应用”、2021.01-2024.12、63万元、在研、主持。 12. 吉林省科技厅重点研发项目，“新型全自动在线药物溶出分析检测技术及其仪器研发”、2020.01-2022.12、30万 元、在研、主持。 11. 国家自然科学基金面上项目，21775017、“口服固体药物制剂体外溶出度的高时间分辨在线过程分析”、2018.01-2021.12、65万元、在研、主持。 10. 国家自然科学基金面上项目，21475019、“基于毛细管电泳技术的微生物胞内目标代谢物在线分析”、2015/01-2018/12、80万元、已结题、主持。 9. 国家自然科学基金面上项目，21175018、“以毛细管电泳为基础的酶切多肽反应全程的高时间分辨检测技术及应用研究”、2012/01-2015/12、56万元、已结题、主持。 8. 吉林省科技厅重点项目，20120431、“基于液液微萃取-高效毛细管电泳技术联用的生物检材中痕量滥用药物的快速灵敏检测”、2012/01-2014/12、15万元、已结题、主持。 7. 东北师范大学“东师学者”青年学术骨干培养计划，2012/01-2014/12、10万元、已结题、主持。 6. 国家自然科学基金青年项目、20805005、“光快门漂白进样-电泳媒介微分析法连用的毛细管电泳在线酶反应研究平台”、2009/01-2011/12、20万元、已结题、主持。 5. 教育部新教师基金、200802001014、“开管毛细管固定化酶反应器与双酶底物循环法联用的毛细管电泳谷氨酸测定平台”、2009/01-2011/12、3.6万元、已结题、主持。 4. 教育部留学回国基金、“利用开管毛细管固定化酶反应器的毛细管电泳法测定痕量谷氨酸”、2009/01-2011/12、3.0万元、已结题、主持。 3. 吉林省科技厅青年基金、20080118、“开管毛细管电色谱测定痕量谷氨酸”、2008/10-2010/10、6万元、已结题、主持。 2. 国家“863”计划课题、2013AA032204、“ 面向公共安全用纳米材料及诊断技术的研发”、2013/12-2016/12、1234万元、在研、课题骨干。 1. 国家科技支撑计划项目子课题、2012BAK02B04、“物证检验与溯源关键技术与装备研发”、2012/2-2014/12、525万元、在研、课题骨干。 主要研究论文 2020年 Immunoassays using optical-fiber sensor with all-directional chemiluminescent collection. Anal. Chem. 2020,92,6257−6262 A portable pencil-like immunosensor for point-of-care testing of inflammatory biomarkers. Anal. Bioanal. Chem. 2020,412,3231-3239 Ultrasensitive determination of organotin compounds in plastic food packaging and edible oils by sheathless capillary electrophoresis-electrospray ionization-mass spectrometry. Analyst 2020,145,2286 Capillary electrophoresis-integrated immobilized enzyme microreactor with graphene oxide as support: Immobilization of negatively charged L-lactate dehydrogenase via hydrophobic interactions. Electrophoresis 2020,41,175-182 模块化自组装高效毛细管电泳教学仪的研制及实验教学应用,化学教育，2020,41,36-40 Capillary electrophoresis-immobilized enzyme microreactors for acetylcholinesterase assay with surface modification by highly-homogeneous microporous layer. J. Chromatogr. A 2020,1609,460454 2019年 Ultra-sensitive capillary immunosensor combining porous-layer surface modification and biotin streptavidin nano-complex signal amplification: Application for sensing of procalcitonin in serum. Talanta 2019,205,120089 Enzyme-like catalysis of polyoxometalates for chemiluminescence: Application in ultrasensitive detection of H2O2 and blood glucose. Talanta 2019,205,120139 Automatic dissolution testing with high-temporal resolution for both immediate-release and fixed-combination drug tablets. Sci.Rep. 2019,205,120139 Sensitive detection of benzophenone-type ultraviolet filters in plastic food packaging materials by sheathless capillary electrophoresis-electrospray ionization-tandem mass spectrometry. J. Chromatogr. A 2019,1604,460469 Optical fiber-mediated immunosensor with a tunable detection range for multiplexed analysis of veterinary drug residues. ACS Sensors 2019,4,1864-1872 A highly sensitive capillary-based immunosensor by combining with peroxidase nano complex-mediated signal amplification for detection of procalcitonin in human serum. ACS Omega 2019,4,6210-6217 Converting solution viscosity to distance-readout on paper substrates based on enzyme-mediated alginate hydrogelation: Quantitative determination of organophosphorus pesticides. Anal. Chim. Acta 2019,1071,1-7 Surface modification with highly-homogeneous porous silica layer for enzyme immobilization in capillary enzyme microreactors. Talanta 2019,197,539-547 2018年 Capillary electrophoresis-integrated immobilized enzyme microreactor utilizing single-step in-situ penicillinase-mediated alginate hydrogelation: Application for enzyme assays of penicillinase. Talanta 2018,189,377-382 Multiplex immunoassays using surface modification-mediated porous layer open tubular capillary. Anal. Chim. Acta 2018,1043,1-10 Enzyme assay for d-amino acid oxidase using optically gated capillary electrophoresis-laser induced fluorescence detection. J. Chromatogr. A 2018,1548,83-91 Single-step in situ acetylcholinesterase-mediated alginate hydrogelation for enzyme encapsulation in CE. Anal. Chem. 2018,90,4071-4078 Rapid and sensitive tapered-capillary microextraction combined to on-line sample stacking-capillary electrophoresis for extraction and quantification of two beta-blockers in human urine. Talanta 2018,180,90-97 Highly uniform porous silica layer open-tubular capillary columns produced via in-situ biphasic sol-gel processing for open-tubular capillary electrochromatography. J. Chromatogr. A 2018,1538,86-93 2017年 Photonic crystal fiber-based immunosensor for high-performance detection of alpha fetoprotein. Biosens. Bioelectron. 2017,91,431-435 Screening platform based on robolid microplate for immobilized enzyme-based assays. ACS Omega 2017,2,5079-5086 Core-shell silica microsphere-based trypsin nanoreactor for low molecular-weight proteome analysis. Anal. Chim. Acta 2017,985,194-201 2016年 Facile synthesis of electrospinning Mn2O3-Fe2O3 loaded carbon fibers for electrocatalysis of hydrogen peroxide reduction and hydrazine oxidation. Electrochim. Acta. 2016,211,255-264 Capillary zone electrophoresis as a tool to monitor the stability and hydrolytic equilibria of strandberg-type polyoxometalates in aqueous solution. Eur. J Inorg. Chem. 2016,4179-4184 Enzyme and inhibition assay of urease by continuous monitoring of the ammonium formation based on capillary electrophoresis. Electrophoresis 2016,37,2692-2698 Sequential on-line C-terminal sequencing of peptides based on carboxypeptidase Y digestion and optically gated capillary electrophoresis with laser-induced fluorescence detection. J. Chromatogr. A 2016,1459,152-159 Capillary electrophoresis-integrated immobilized enzyme reactors. Rev. Anal. Chem. 2016,35,115-131 Determination of the inhibitory effect of green tea extract on glucose-6-phosphate dehydrogenase based on multilayer capillary enzyme microreactor. Biomed. Chromatogr. 2016,30,1210-1215 2015年 Sequential capillary electrophoresis analysis using optically gated sample injection and UV/vis detection. Electrophoresis 2015,36,2380-2385 Enhancing separation in short-capillary electrophoresis via pressure-driven backflow. Electrophoresis 2015,36,1549-1554 On-plate enzyme and inhibition assay of glucose-6-phosphate dehydrogenase using thin-layer chromatography. J. Sep. Sci. 2015,38,2753-2930 Application of Thin-Layer Chromatography in Enzyme Activity and Inhibitors Studies of Glucose-6-Phosphate Dehydrogenase. JPC-J. Planar Chromat. 2015,28,333-336 Application of capillary enzyme micro-reactor in enzyme activity and inhibitors studies of glucose-6-phosphate dehydrogenase. J. Chromatogr. B 2015,990,174-180 Theoretical and experimental studies on sequential two-diffusional sample injection for capillary electrophoresis. J. Chromatogr. A 2015,138,247-252 2014年 Inhibition study of alanine aminotransferase enzyme using sequential online capillary electrophoresis analysis. Anal. Biochem. 2014,467,28-30 Capillary electrophoresis-based immobilized enzyme reactor using particle-packing technique. J. Chromatogr. A 2014,1352,80-86 A capillary electrophoresis-based immobilized enzyme reactor using graphene oxide as a support via layer by layer electrostatic assembly. Analyst 2014,139,1973 Efficient capillary electrophoresis separation and determination of free amino acids in beer samples. Electrophoresis 2014,35,577-584 Sequential micellar electrokinetic chromatography analysis of racemization reaction of alanine enantiomers. J. Chromatogr. A 2014,1331,123-128 2013年 Ionic liquid-based dispersive liquid-liquid microextraction coupled with capillary electrophoresis to determine drugs of aAbuse in urine. Chinese J. Anal. Chem. 2013,41,1919-1922 Graphene oxide and reduced graphene oxide as novel stationary phases via electrostatic assembly for open-tubular capillary electrochromatography. Electrophoresis 2013,34,1869-1876 Application of cyclodextrin-modified gold nanoparticles in enantioselective monolith capillary electrochromatography. Talanta 2013,109,1-6 Application of graphene as the stationary phase for open-tubular capillary electrochromatography.J. Chromatogr. A 2013,1277,93-97 2012年 Electrochemistry and simultaneous detection of metabolites of purine nucleotide based on large mesoporous carbon modified electrode. Electroanal. 2012,24,1401-1408 Online enzyme discrimination and determination of substrate enantiomers based on electrophoretically mediated microanalysis. Anal. Chem. 2012,84,6701-6706 Method for the sequential online analysis of enzyme reactions based on capillary electrophoresis. Anal. Chem. 2012,84,2961-2967 A replaceable dual-enzyme capillary microreactor using magnetic beads and its application for simultaneous detection of acetaldehyde and pyruvate. Electrophoresis 2012,33,2145-2151 2011年 Enantioselective open-tubular capillary electrochromatography using cyclodextrin-modified gold nanoparticles as stationary phase. J. Chromatogr. A 2011,1218,3725-3729 爆炸残留物的毛细管电泳检测。分析化学 2011,39,1293-1294 2010年 Use of cyclodextrin-modified gold nanoparticles for enantioseparations of drugs and amino acids based on pseudostationary phase-capillary electrochromatography. Electrophoresis 2010,31,1-9 High temporal resolution monitoring of enzyme reaction and inhibition using optically gated vacancy capillary electrophoresis and immobilized enzyme. Anal. Chim. Acta 2010,683,136-142 2009年 Dual-enzyme, co-immobilized capillary microreactor combined with substrate recycling for high-sensitive glutamate determination based on CE. Electrophoresis 2009,30,3527-3533